LSBio
LIN28B Antibody (FITC)

LIN28B Antibody (FITC)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay
Reactivity:Human
Format:Liquid
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$300.00each
Catalog Number: LS-C501077-50

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Overview

Description
LIN28B antibody LS-C501077 is an FITC-conjugated rabbit polyclonal antibody to human LIN28B. Validated for ELISA.
Application
ELISA
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CSDD2 | Lin-28B | LIN28B | Lin-28 homolog B (C. elegans) | Protein lin-28 homolog B | Lin-28.2
UniProt Number
Q6ZN17
NCBI GenBank
NP_001004317.1
SwissProt
Q6ZN17

Target

Target
Human LIN28B
Antigen Species
Human
Epitope
Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PubMed:22118463). Does not act on pri-miR21 (PubMed:22118463). The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PubMed:19703396). This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent (PubMed:22118463). Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity). When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
Gene Name
LIN28B

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Recombinant Human Protein lin-28 homolog B protein (166-250AA)
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Fluorescein Isothiocyanate
Format
Liquid
Formulation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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