LSBio
IHC-plus™ Polyclonal Rabbit anti-Human LIN28B Antibody (aa1-30, IHC, IF, WB)

Anti-LIN28B antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ Polyclonal Rabbit anti-Human LIN28B Antibody (aa1-30, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$470.00each
Catalog Number: LS-B3423-200

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Overview

Description
LIN28B antibody LS-B3423 is an unconjugated rabbit polyclonal antibody to human LIN28B (aa1-30). Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Application
FC, IF, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CSDD2 | Lin-28B | LIN28B | Lin-28 homolog B (C. elegans) | Protein lin-28 homolog B | Lin-28.2
Recommended Dilution: FC
1:10 - 1:50
Recommended Dilution: IF/ICC
1:100
Recommended Dilution: IHC-P
10 µg/ml
Recommended Dilution: WB
1:1000
UniProt Number
Q6ZN17
NCBI GenBank
NP_001004317.1
SwissProt
Q6ZN17

Target

Target
LIN28B
Antigen Species
Human
Specificity
aa1-30 of human LIN28B
Epitope
This LIN28B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LIN28B.
Gene Name
LIN28B

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Volume
200 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Recommended Storage Buffer
PBS

Lin28A and Lin28B inhibit let-7 microRNA biogenesis by distinct mechanisms. Piskounova E, Polytarchou C, Thornton JE, LaPierre RJ, Pothoulakis C, Hagan JP, Iliopoulos D, Gregory RI. Cell. 2011 Nov;147:1066-79.

PubMed: 22118463 · PMC: PMC3227872


Lin28B-high breast cancer cells promote immune suppression in the lung pre-metastatic niche via exosomes and support cancer progression. Meiyan Qi , Yun Xia , Yanjun Wu, Zhuo Zhang, Xinyu Wang, Liying Lu, Cheng Dai, Yanan Song, Keying Xu, Weiwei Ji, Lixing Zhan. Nature communications. 2022 February;13:897.

PubMed: 35173168 · PMC: PMC8850492

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