Induction of LIME tyrosine phosphorylation. Peripheral blood T cells were left unstimulated (-) or stimulated with anti-human CD3 (MEM-92) or anti-human CD4 (MEM-16), and LIME was immunoprecipitated from laurylmaltoside lysates with the LIME-06 antibody (immunoaffinity sorbent) and analyzed by Western blotting to visualize tyrosine-phosphorylated LIME (top) and total LIME (bottom).
LIME1 / LIME Antibody (aa141-295, clone LIME-06)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunohistochemistry (general), Immunoprecipitation |
| Reactivity: | Human |
| Format: | Liquid |
$344.00each
Catalog Number: LS-C46068-0.1
Catalog Number: LS-C46068
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- Description
- LIME antibody LS-C46068 is an unconjugated mouse monoclonal antibody to human LIME (LIME1) (aa141-295). Validated for IP.
- Application
- IHC, IP
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- DJ583P15.4 | LIME1 | Lck-interacting molecule | LIME | RP4-583P15.5 | LP8067
- UniProt Number
- Q9H400
- NCBI GenBank
- NM_017806 | NP_060276.2
- SwissProt
- Q9H400
Target
- Target
- Human LIME1 / LIME
- Antigen Species
- Human
- Epitope
- The antibody LIME-06 was raised against intracellular fragment corresponding to aa 141-295 of human LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.
- Gene Name
- LIME1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- LIME-06
- Isotype
- IgG1
Immunogen
- Immunogen
- Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME
- Immunogen Type
- Cell-Based Immunization
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 15 mM Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C. Do not freeze.
- Recommended Storage Buffer
- PBS