Immunofluorescence Microscopy of Biotin conjugated Anti-Lactate Dehydrogenase Antibody. Tissue: HeLa cells. Fixation: fixed for 5 min in 1:1 MeTOH:Acetone, blocked with MB-071 (preservative free) for 15 min. Antigen retrieval: not required. Primary antibody: Lactate Dehydrogenase antibody at 1:200 for 1 h at RT. Secondary antibody: DyLight 488 conjugated Streptavidin antibody at 1:10,000 for 30 min at RT. Staining: Lactate Dehydrogenase as green fluorescent signal.
LDHA / LDH1 Antibody (Lyophilized)
Primary AntibodyLSBio Guarantee
| Antibody: | Goat Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunoprecipitation, Western Blot |
| Reactivity: | Rabbit |
| Format: | Lyophilized |
$579.00each
Catalog Number: LS-C209189-100
Catalog Number: LS-C209189
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- Description
- LDH1 antibody LS-C209189 is an unconjugated goat polyclonal antibody to rabbit LDH1 (LDHA). Validated for ELISA, IF, IP and WB.
- Application
- ELISA, IF, IP, WB
- Reactivity
- Rb
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- LDH muscle subunit | LDH1 | LDHA | Lactate dehydrogenase M | LDH-M | GSD11 | Lactate dehydrogenase A | LDH-A | LDHM | PIG19 | Proliferation-inducing gene 19
- Recommended Dilution: ELISA
- 1:2000 - 1:10000
- Recommended Dilution: IF/ICC
- 1:200
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:1000 - 1:5000
- UniProt Number
- P00338
- NCBI GenBank
- NM_005566 | NP_005557.1
- SwissProt
- P00338
Target
- Target
- Rabbit LDHA / LDH1
- Antigen Species
- Rabbit
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]. BLAST analysis was used to determine that cross reactivity is suggested for both muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species.
- Gene Name
- LDHA
Antibody
- Host Species
- Goat
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This antibody was prepared from whole goat serum produced by repeated immunizations with a full length lactate dehydrogenase protein isolated from rabbit muscle.
- Immunogen Type
- Native Protein (Purified from Natural Source)
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl