LSBio
Monoclonal Mouse anti-Human LDHA / LDH1 Antibody (clone OTI2D11, IHC, WB)

Immunoprecipitation(IP) of LDHA by using monoclonal anti-LDHA antibodies (Negative control: IP without adding anti-LDHA antibody.). For each experiment, 500ul of DDK tagged LDHA overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-LDHA antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Monoclonal Mouse anti-Human LDHA / LDH1 Antibody (clone OTI2D11, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
See all Human LDHA / LDH1 antibodies →
$443.00each
Catalog Number: LS-C114665-100

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Overview

Description
LDH1 antibody LS-C114665 is an unconjugated mouse monoclonal antibody to human LDH1 (LDHA). Validated for IHC, IP and WB.
Application
IHC, IHC-P, IP, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
LDH muscle subunit | LDH1 | LDHA | Lactate dehydrogenase M | LDH-M | GSD11 | Lactate dehydrogenase A | LDH-A | LDHM | PIG19 | Proliferation-inducing gene 19
Recommended Dilution: IHC-P
1:50
Recommended Dilution: WB
1:1000
UniProt Number
P00338
SwissProt
P00338

Target

Target
Human LDHA / LDH1
Antigen Species
Human
Epitope
Human LDHA / Lactate Dehydrogenase A
Gene Name
LDHA

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
OTI2D11
Isotype
IgG1

Immunogen

Immunogen
Full length human recombinant protein of human LDHA (NP_005557) produced in HEK293T cell.
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Concentration
1 mg/mL
Volume
100 Microliter
Preservative
Yes
Purification Method
Protein A/G Affinity

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.