LSBio
Kinesin Heavy Chain / KIF5B Antibody

Western Blot of Mouse Anti-AKT3 antibody. Lane 1: C2C12. Lane 2: MEF#1. Lane 3: MEF#2. Lane 4: A549. Lane 5: Calu-1. Lane 6: PC3. Lane 7: HepG2. Lane 8: Jurkat. Lane 9: SKOV3. Lane 10: 293T. Load: 35 µg per lane. Primary antibody: AKT-3 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Anti mouse secondary antibody at 1:20,000 for 1 h at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT3.

Kinesin Heavy Chain / KIF5B Antibody

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Liquid
See all Human Kinesin Heavy Chain / KIF5B antibodies →
$311.00each
Catalog Number: LS-C744603-25

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Overview

Description
KIF5B antibody LS-C744603 is an unconjugated rabbit polyclonal antibody to human KIF5B (Kinesin Heavy Chain). Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Kinesin 1 (110-120kD) | KINH | KNS | Kinesin family member 5B | Kinesin heavy chain | KNS1 | UKHC | KIF5B | Kinesin-1 heavy chain | Ubiquitous kinesin heavy chain
Recommended Dilution: ELISA
1:10000
Recommended Dilution: WB
1:100 - 1:500
UniProt Number
P33176
NCBI GenBank
NM_004521 | NP_004512.1
SwissProt
P33176

Target

Target
Human Kinesin Heavy Chain / KIF5B
Antigen Species
Human
Epitope
A BLAST analysis was used to suggest reactivity with human. Cross-reactivity with kinesin 1 from other sources have not been determined.
Gene Name
KIF5B

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Anti-Kinesin-1 was prepared from whole rabbit serum produced by repeated immunizations with a truncated kinesin-1 construct expressed in E. coli corresponding to human kinsesin-1 protein.
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
77 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
Other

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.