LSBio
Kinesin Heavy Chain / KIF5B Antibody

Western blot of Rabbit anti-Kinesin 1 Antibody. Lane 1: 500 ng of truncated kinesin 1 protein. Lane 2: 20 ug of Mouse Brain lysate. Lane 3: 10 ug of Mouse Brain lysate. Primary antibody: kinesin1 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:40000 for 1hr at RT. Block: 5% BLOTTO 1 hr at RT. Predicted/Observed size: 72 kDa, ~70 kDa for kinesin-1. Other band(s): degradation.

Kinesin Heavy Chain / KIF5B Antibody

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Liquid
See all Human Kinesin Heavy Chain / KIF5B antibodies →
$579.00each
Catalog Number: LS-C153763-100

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Overview

Description
KIF5B antibody LS-C153763 is an unconjugated rabbit polyclonal antibody to human KIF5B (Kinesin Heavy Chain). Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Kinesin 1 (110-120kD) | KINH | KNS | Kinesin family member 5B | Kinesin heavy chain | KNS1 | UKHC | KIF5B | Kinesin-1 heavy chain | Ubiquitous kinesin heavy chain
Recommended Dilution: ELISA
1:10000
Recommended Dilution: WB
1:100 - 1:500
UniProt Number
P33176
SwissProt
P33176

Target

Target
Human Kinesin Heavy Chain / KIF5B
Antigen Species
Human
Epitope
A BLAST analysis was used to suggest reactivity with human. Cross-reactivity with kinesin 1 from other sources have not been determined.
Gene Name
KIF5B

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
Anti-Kinesin-1 was prepared from whole rabbit serum produced by repeated immunizations with a truncated kinesin-1 construct expressed in E. coli corresponding to human kinsesin-1 protein.
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
77 mg/mL
Volume
100 Microliter
Preservative
Yes
Purification Method
Other

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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