Dog peripheral blood monocytes stained with Mouse anti-Dog CD18 followed by Rabbit F(ab')2 anti-Mouse IgG:FITC
ITGB2 / CD18 Antibody (clone CA1.4E9)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunoprecipitation |
| Reactivity: | Dog |
| Format: | Liquid |
$304.00each
Catalog Number: LS-C187474-0.1
Catalog Number: LS-C187474
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- Description
- CD18 antibody LS-C187474 is an unconjugated mouse monoclonal antibody to dog CD18 (ITGB2). Validated for Flow, IF, IHC and IP.
- Application
- FC, IF, IHC, IHC-F, IP
- Reactivity
- Can
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- CD18 antigen | CD18 | Integrin beta chain, beta 2 | ITGB2 | LCAMB | MAC-1 | MFI7 | MF17 | Integrin beta-2 | LAD | LFA-1
- UniProt Number
- P05107
- NCBI GenBank
- NM_000211 | NP_000202.3
- SwissProt
- P05107
Target
- Target
- Dog ITGB2 / CD18
- Antigen Species
- Dog
- Epitope
- Mouse anti Dog CD18 antibody, clone CA1.4E9 recognizes the canine CD18 cell surface antigen, also known as the b2 integrin. The tissue and cellular distribution of CD18 in canine tissue closely follows that observed for humans CD18 is expressed by virtually all leucocytes, but more strongly upon monocytes and granulocytes than on lymphocytes. The cross reactivity patterns of Mouse anti Canine CD18, clone CA1.4E9 further indicate that the antibody clone recognizes an epitope common to a number of mammalian species. Immunoprecipitation experiments using detergent lysates of iodinated peripheral blood leukocytes indicate that clone Ca1.4E9 immunoprecipitates the common 95kD beta 2 integrin chain (CD18) along with the non-covalently associated alpha chains at 180kD (CD11a ), 165kD (CD11b) and 150kD (CD11c). Of note is the lack of precipitation of the integrin alpha D chain (CD11d), this is likely due to the almost complete absence of CD11d expression on peripheral blood lymphocytes, in contrast to expression in the splenic red pulp. High CD18 expression is a common feature of lymphoma and hyperplasia in dogs.
- Gene Name
- ITGB2
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- CA1.4E9
- Isotype
- IgG1
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
- Recommended Storage Buffer
- PBS