Western blot using the Protein-A Purified Anti-bovine IL-21 antibody shows detection of recombinant bovine IL-21 at 15.1kDa (arrow) raised in yeast. Protein was purified and resolved by SDS-PAGE, transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with Anti-bovine IL-21. After washing, membrane was probed with Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) antibody.

IL21 Antibody (aa24-152, Liquid)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:	Bovine
Format:	Liquid

$311.00each

Catalog Number: LS-C744976-25

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: IL21 antibody LS-C744976 is an unconjugated rabbit polyclonal antibody to IL21 (aa24-152) from bovine. It is reactive with bovine, horse and pig. Validated for ELISA and WB.
Application: ELISA, WB
Reactivity: Bov
Predicted Reactivity: Horse, Pig
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: IL21 | Interleukin 21 | Interleukin-21 | Interleukin-21 isoform | Za11 | IL-21
Recommended Dilution: ELISA: 1:6000
Recommended Dilution: WB: 1 µg/ml
UniProt Number: Q9HBE4
NCBI GenBank: NM_021803 | NP_068575.1
SwissProt: Q9HBE4

Target

Target: Bovine IL21
Antigen Species: Bovine
Epitope: This antibody is specific for bovine IL-21 protein. A BLAST analysis was used to suggest cross-reactivity with IL-21 from bovine sources based on 100% homology with the immunizing sequence. Based on 92% homology, there is a chance of cross-reactivity to porcine IL- 21, 91% to horse IL-21, 89% to human and dog, 88% to macaque, 84% to platypus and Syrian hamster, 76-79% to mouse, 75% to rat, and 48% to chicken. Cross-reactivity with IL-21 from other sources has not been determined.
Gene Name: IL21

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to amino acid residues 24-152 of bovine IL-21 protein.
Immunogen Type: Recombinant Protein Fragment

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Volume: 25 Microliter
Preservative: Yes
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl