Mouse IL1A was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate. 1 uL anti-Mouse IL1A rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-Mouse IL1A rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 36 kDa.
IL1A / IL-1 Alpha Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot |
| Reactivity: | Mouse |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C818565-100
Catalog Number: LS-C818565
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- Description
- IL-1 Alpha antibody LS-C818565 is an unconjugated rabbit polyclonal antibody to mouse IL-1 Alpha (IL1A). Validated for ELISA, IP and WB.
- Application
- ELISA, IP, WB
- Reactivity
- Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Hematopoietin-1 | IL-1 alpha | IL1 | IL1-ALPHA | IL1 Alpha | Interleukin-1 alpha | IL1A | IL-1A | IL1F1 | Pro-interleukin-1-alpha | Preinterleukin 1 alpha | Interleukin 1, alpha
- Recommended Dilution: ELISA
- 1:25000 - 1:50000
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P01583
- NCBI GenBank
- NM_000575 | NP_000566.3
- SwissProt
- P01583
Target
- Target
- Mouse IL1A / IL-1 Alpha
- Antigen Species
- Mouse
- Epitope
- Mouse IL-1 alpha/IL1A
- Gene Name
- IL1A
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Mouse IL1A / IL-1A / IL-1F1 Protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS