Fig-1: Intracellular staining of IL-17A in PBMC treated with PMA (25ng/ml) and ionomycin (250ng/ml) for overnight, then Brefedin A (1x) was added to cells for 4hr prior to harvest. Anti-IL-17A was used at 2 µg/10^6 cells. Green represents isotype control; red represents Anti-IL-17A. Goat anti-mouse PE conjugate was used as secondary antibody.

IL17A Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Flow Cytometry
Reactivity:	Human
Format:	Liquid

$294.00each

Catalog Number: LS-C783132-25

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Quantity

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Overview

Description: IL17A antibody LS-C783132 is an unconjugated mouse monoclonal antibody to human IL17A. Validated for Flow.
Application: FC
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: CTLA-8 | CTLA8 | IL17A | Interleukin-17A | Interleukin 17A | IL-17 | IL-17A | IL17
Recommended Dilution: FC: 2 - 4 µg/10E6 cells
UniProt Number: Q16552
NCBI GenBank: NM_002190 | NP_002181.1
SwissProt: Q16552

Target

Target: Human IL17A
Antigen Species: Human
Gene Name: IL17A

Antibody

Host Species: Mouse
Clonality: Monoclonal
Isotype: IgG2a
Light Chain: Kappa Light Chain
Origin Species: Human

Immunogen

Immunogen: Full length recombinant protein of Interleukin-17A
Immunogen Type: Full-Length Recombinant Protein

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS with 0.05% BSA and 0.05% sodium azide.
Concentration: 0.5 mg/mL
Preservative: Yes
Purification Method: Protein G Affinity

Storage & Handling

Storage Conditions: Store at 4°C for up to 6 months. For long-term storage, store at -20°C. Avoid freeze/thaw cycles.
Recommended Storage Buffer: PBS