Anti-Human IL17-A Antibody - Western Blot. Western blot of anti-Human IL17-A antibody shows detection of a band ~17 kD in size corresponding to recombinant human IL17-A (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1000 dilution. Detection occurred using DyLight 649 conjugated anti-Rabbit IgG ( secondary antibody diluted 1:20000 in blocking buffer (p/n MB-070). Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).

IL17A Antibody (Lyophilized)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:	Human
Format:	Lyophilized

$579.00each

Catalog Number: LS-C154082-100

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Quantity

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Overview

Description: IL17A antibody LS-C154082 is an unconjugated rabbit polyclonal antibody to human IL17A. Validated for ELISA, IHC and WB.
Application: ELISA, IHC, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: CTLA-8 | CTLA8 | IL17A | Interleukin-17A | Interleukin 17A | IL-17 | IL-17A | IL17
Recommended Dilution: ELISA: 1:1000 - 1:5000
Recommended Dilution: IHC: 1:100
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: Q16552
NCBI GenBank: NM_002190 | NP_002181.1
SwissProt: Q16552

Target

Target: Human IL17A
Antigen Species: Human
Epitope: This antibody will recognize both native and recombinant human IL-17A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended. Cross-reactivity with IL-17 from other sources has not been determined.
Gene Name: IL17A

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: IL-17A Antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL17-A protein.
Immunogen Type: Full-Length Recombinant Protein

Format

Conjugate/Label: Unconjugated
Format: Lyophilized
Formulation: Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl