LSBio
IHC-plus™ VTN / Vitronectin Antibody (aa1-310, clone 6F212)

Anti-VTN / Vitronectin antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ VTN / Vitronectin Antibody (aa1-310, clone 6F212)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Bovine, Dog, Cat, Human, Rabbit
Format:Liquid
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$516.00each
Catalog Number: LS-B10373-50

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Overview

Description
Vitronectin antibody LS-B10373 is an unconjugated mouse monoclonal antibody to Vitronectin (VTN) (aa1-310) from human. It is reactive with human, bovine, cat and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Bov, Can, Fel, Hu, Rb
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
Epibolin | Serum-spreading factor | VNT | VTN | S-protein | Somatomedin B | V75 | VN | Complement S-protein | Serum spreading factor | Vitronectin
Recommended Dilution: ELISA
1:40000
Recommended Dilution: IHC-P
10 µg/ml
Recommended Dilution: WB
1:50
UniProt Number
P04004
SwissProt
P04004

Target

Target
VTN / Vitronectin
Antigen Species
Human
Specificity
Highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). Cross-reacts with vitronectin from cat, dog, rabbit and to a lesser extent with cow. Epitope is located within a cyanogen bromide cleavage fragment comprising aa 1-310. Binds nearly as well to native vitronectin as to denatured. It interferes with vitronectin-inhibition of the lysis of terminal complement complexes (TCC) and it inhibits vitronectin binding of PAI-1.
Epitope
Highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). Cross-reacts with vitronectin from cat, dog, rabbit and to a lesser extent with cow. Epitope is located within a cyanogen bromide cleavage fragment comprising aa 1-310. Binds nearly as well to native vitronectin as to denatured. It interferes with vitronectin-inhibition of the lysis of terminal complement complexes (TCC) and it inhibits vitronectin binding of PAI-1.
Gene Name
VTN

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
6F212
Light Chain
Kappa Light Chain
Origin Species
Human

Immunogen

Immunogen
Human vitronectin purified from plasma by heparin-affinity chromatography.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 15 mM Sodium Azide
Concentration
1 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein A/G Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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