Anti-UNG antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ UNG / Uracil DNA Glycosylase Antibody (aa281-298)

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Antibody:	Rabbit Polyclonal
Applications:	Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Human
Format:	Liquid

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$383.00each

Catalog Number: LS-B6459-50

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Overview

Description: Uracil DNA Glycosylase antibody LS-B6459 is an unconjugated rabbit polyclonal antibody to human Uracil DNA Glycosylase (UNG) (aa281-298). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application: IHC, IHC-P, WB
Reactivity: Hu
Predicted Reactivity: Bovine, Dog, Chicken, Horse, Mammal (Other), Mouse, Non-Human Primate, Rabbit
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: DGU | HIGM4 | HIGM5 | Uracil-DNA glycosylase | UNG1 | UNG2 | UDG | UNG | UNG15
Recommended Dilution: IHC-P: 10 µg/ml
Recommended Dilution: WB: 2 µg/ml
UniProt Number: P13051
NCBI GenBank: NM_003362 | NP_003353.1
SwissProt: P13051

Target

Target: UNG / Uracil DNA Glycosylase
Antigen Species: Human
Specificity: A synthetic peptide sequence CRHFSKTNELLQKSGKKP corresponding to amino acids 281-298 of human UNG1 (NP003353.1) and amino acids 290-307 of human UNG2 (NP 550433.1). The peptide sequence used for immunogen is highly conserved between mammalian and invertabrates.
Epitope: The synthetic peptide sequence used for immunogen is 100% conserved between UNG1 and UNG2. Thus recognizes both UNG1 and UNG2. As UNG1 and UNG2 are not resolved as two species in gel electrophoresis (Huag et al., 1998), they are indistinguishable by western blot. The observed molecular weight of UNG (UNG1 and UNG2) on western blots may vary: 26 kD, 27.5 kD, 31 kD, and 38 kD forms have been described (Bharati et al., 1998; Slupphaug et al., 1993). Please see Kruman et al. (Fig. 1D, 2004) for an example of western blot in rat. This specificity of this has been validated by antisense UNG oligonucleotide (Kruman et al. 2004).
Gene Name: UNG

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Origin Species: Human

Immunogen

Immunogen: Synthetic peptide sequence CRHFSKTNELLQKSGKKP corresponding to amino acids 281-298 of human UNG1 (NP003353.1) and amino acids 290-307 of human UNG2 (NP 550433.1). The peptide sequence used for immunogen is highly conserved between mammalian and invertabrates. Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%); Gibbon, Marmoset, Mouse, Dog, Bat, Bovine, Horse, Rabbit, Turkey, Chicken (94%); Rat, Hamster, Elephant, Panda, Pig, Platypus (89%); Zebrafish (83%).
Immunogen Type: Synthetic Peptide (Linear)