Western blot analysis of extracts of various cell lines, using UNG antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
UNG / Uracil DNA Glycosylase Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C748216-20
Catalog Number: LS-C748216
For bulk orders or custom formulations:
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- Description
- Uracil DNA Glycosylase antibody LS-C748216 is an unconjugated rabbit polyclonal antibody to Uracil DNA Glycosylase (UNG) from human. It is reactive with human, mouse and rat. Validated for WB.
- Application
- WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- DGU | HIGM4 | HIGM5 | Uracil-DNA glycosylase | UNG1 | UNG2 | UDG | UNG | UNG15
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P13051
- NCBI GenBank
- NM_003362 | NP_003353.1
- SwissProt
- P13051
Target
- Target
- Human UNG / Uracil DNA Glycosylase
- Antigen Species
- Human
- Gene Name
- UNG
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UNG (NP_550433.1). MIGQKTLYSFFSPSPARKRHAPSPEPAVQGTGVAGVPEESGDAAAIPAKKAPAGQEEPGTPPSSPLSAEQLDRIQRNKAAALLRLAARNVPVGFGESWKK
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS