LSBio
IHC-plus™ Polyclonal Rabbit anti-Human CKS1B / CKS1 Antibody (C-Terminus, IHC, WB)

Anti-CKS1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.

IHC-plus™ Polyclonal Rabbit anti-Human CKS1B / CKS1 Antibody (C-Terminus, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
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$495.00each
Catalog Number: LS-B8108-50

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Overview

Description
CKS1 antibody LS-B8108 is an unconjugated rabbit polyclonal antibody to human CKS1 (CKS1B) (C-Terminus). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IHC, IHC-P, IP, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CDC28 protein kinase 1 | CKS-1 | CDC28 protein kinase 1B | CKS1B | Ckshs1 | PNAS-18 | PNAS-143 | PNAS-16 | CDC2-associated protein CKS1 | CKS1
Recommended Dilution: ELISA
0.1 - 1 µg/ml
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
1 - 3 µg/ml
UniProt Number
P61024
SwissProt
P61024

Target

Target
CKS1B / CKS1
Antigen Species
Human
Specificity
Reacts with the human Cks1 protein. On Western blots, it identifies a single band at ~11kD. Bands of unknown origin at ~20, 30 and 100kD are also observed. Reactivity has been confirmed with HeLa cell lysates by Western blotting, immunofluorescence and immunoprecipitation, and with non-small cell lung carcinoma tissue by immunohistochemistry. Western blotting with purified Cks1 and Cks2 proteins was used to confirm that this antibody does not cross-react with Cks2. Cross-reactivity with mouse is expected based upon amino acid sequence homology.
Epitope
Reacts with the human Cks1 protein. On Western blots, it identifies a single band at ~11kD. Bands of unknown origin at ~20, 30 and 100kD are also observed. Reactivity has been confirmed with HeLa cell lysates by Western blotting, immunofluorescence and immunoprecipitation, and with non-small cell lung carcinoma tissue by immunohistochemistry. Western blotting with purified Cks1 and Cks2 proteins was used to confirm that this antibody does not cross-react with Cks2. Cross-reactivity with mouse is expected based upon amino acid sequence homology.
Gene Name
CKS1B

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Synthetic peptide derived from the C-Terminus of human and mouse Cks1.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 0.09% Sodium Azide
Concentration
0.25 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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