LSBio
IHC-plus™ Alpha Tubulin Antibody (C-Terminus, clone YL1/2)

Anti-Alpha Tubulin antibody IHC of human brain, cerebellum, Purkinje cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ Alpha Tubulin Antibody (C-Terminus, clone YL1/2)

Primary AntibodyLSBio Guarantee
Antibody:Rat Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Radioimmunoassay, Western Blot
Reactivity:Other
Format:Liquid
See all Alpha Tubulin antibodies →
$536.00each
Catalog Number: LS-B6777-50

For bulk orders or custom formulations:

Request a Quote

Overview

Description
Alpha Tubulin antibody LS-B6777 is an unconjugated rat monoclonal antibody to yeast Alpha Tubulin (C-Terminus). Validated for ELISA, IF, IHC, IP, RIA and WB.
Application
ELISA, IF, IHC, IHC-F, IHC-P, IP, RIA, WB
Reactivity
Other
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: ELISA
1:100 - 1:1000
Recommended Dilution: IHC-P
5 µg/ml

Target

Target
Alpha Tubulin
Antigen Species
Other
Specificity
Recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin. This product is routinely tested in ELISA on Tubulin
Epitope
Recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin. This product is routinely tested in ELISA on Tubulin.

Antibody

Host Species
Rat
Clonality
Monoclonal
Clone
YL1/2

Immunogen

Immunogen
Yeast tubulin.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.