LSBio
IHC-plus™ PCNA Antibody

Anti-PCNA antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ PCNA Antibody

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Format:Liquid
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$526.00each
Catalog Number: LS-B402-50

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Overview

Description
PCNA antibody LS-B402 is an unconjugated rabbit polyclonal antibody to PCNA from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IHC, IHC-P, WB
Predicted Reactivity
Bovine, Dog, Chicken, Fish (Other), Human, Mouse, Non-Human Primate, Rat, Xenopus
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Cyclin | PCNA
Recommended Dilution: ELISA
1:2000 - 1:8000
Recommended Dilution: IHC-P
2.5 µg/ml
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P12004
SwissProt
P12004

Target

Target
PCNA
Antigen Species
Human
Specificity
A BLAST analysis was used to suggest cross reactivity with PCNA protein from human, monkey, dog, mouse, rat, bovine, chicken, fish and Xenopus based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Epitope
A BLAST analysis was used to suggest cross reactivity with PCNA protein from human, monkey, dog, mouse, rat, bovine, chicken, fish and Xenopus based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Gene Name
PCNA

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PCNA protein.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1.69 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Aliquot and store at -20°C or below for extended storage. Avoid freeze / thaw cycles. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.