LSBio
IHC-plus™ Monoclonal Mouse anti-Human SRSF1 / SF2 Antibody (N-Terminus, IHC, IF, WB)

Anti-SF2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ Monoclonal Mouse anti-Human SRSF1 / SF2 Antibody (N-Terminus, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
See all SRSF1 / SF2 antibodies →
$495.00each
Catalog Number: LS-B3263-50

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Overview

Description
SF2 antibody LS-B3263 is an unconjugated mouse monoclonal antibody to SF2 (SRSF1) (N-Terminus) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
IF, IHC, IHC-P, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
Alternative-splicing factor 1 | ASF | SF2 | SF2p33 | SFRS1 | Splicing factor 2 | ASF-1 | SR splicing factor 1 | SRp30a | SRSF1
Recommended Dilution: IHC-P
10 µg/ml
Recommended Dilution: WB
1 - 3 µg/ml
UniProt Number
Q07955
SwissProt
Q07955

Target

Target
SRSF1 / SF2
Antigen Species
Human
Specificity
Specific for the N-terminal portion (aa1-97) of the SF2/ASF protein comprising the RNA-recognition motif (RRM1). Reactivity is not affected by phosphorylation state of SF2/ASF. On Western blots, a band of ~33kD is seen with doublet or triplet bands sometimes evident due to phosphorylation differences. SF2/ASF is highly conserved (e. g. mouse and human protein are 100% identical) so reactivity with other species is likely. Please note, however, that cross-reactivity in non-target species varies and cannot be guaranteed.
Epitope
Specific for the N-terminal portion (aa1-97) of the SF2/ASF protein comprising the RNA-recognition motif (RRM1). Reactivity is not affected by phosphorylation state of SF2/ASF. On Western blots, a band of ~33kD is seen with doublet or triplet bands sometimes evident due to phosphorylation differences. SF2/ASF is highly conserved (e. g. mouse and human protein are 100% identical) so reactivity with other species is likely. Please note, however, that cross-reactivity in non-target species varies and cannot be guaranteed.
Gene Name
SRSF1

Antibody

Host Species
Mouse
Clonality
Monoclonal
Origin Species
Human

Immunogen

Immunogen
Recombinant SF2/ASF protein.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 0.1% Sodium Azide
Concentration
0.5 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.