Monoclonal Mouse Biotin Antibody
Catalog Number: LS-C127042-200
200 µg / $755.00
Antibody: Mouse Monoclonal
Applications: Enzyme-Linked Immunosorbent Assay
Format: Liquid
Anti-MKI67 / Ki-67 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC-plus™ Monoclonal Mouse anti-Human MKI67 / Ki67 Antibody (clone Ki-S5, IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunocytochemistry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$495.00each
Catalog Number: LS-B6433-50
Catalog Number: LS-B6433
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- Description
- Ki67 antibody LS-B6433 is an unconjugated mouse monoclonal antibody to human Ki67 (MKI67). Validated for Flow, ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
- Application
- FC, ICC, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Antigen KI-67 | Ki67 | KIA | MKI67
- Recommended Dilution: IF/ICC
- 5 - 10 µg/ml
- Recommended Dilution: IHC-P
- 10 µg/ml
- Recommended Dilution: WB
- 1:500
- UniProt Number
- P46013
- NCBI GenBank
- NM_002417 | NP_002408.3
- SwissProt
- P46013
Target
- Target
- MKI67 / Ki67
- Antigen Species
- Human
- Specificity
- Human MKI67
- Epitope
- Recognizes a cell-cycle-associated protein of 345kD and 395kD identical with the human Ki-67 antigen. Binds to a formalin-resistant epitope of the Ki-67 antigen. Confined to the nuclei of proliferating cells and no cross-reactivity with cytoplasmic antigens of epithelial occurs even after antigen retrieval. Immunohistochemistry labeling of fresh and frozen samples showed identical results with Ki-67 and Ki-S5.
- Gene Name
- MKI67
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- Ki-S5
- Origin Species
- Human
Immunogen
- Immunogen
- Nuclear protein preparation from the human cell line U937.50.
- Immunogen Type
- Cell-Based Immunization
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.6, 0.09% Sodium Azide
- Concentration
- 1 mg/mL
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
Targeting stanniocalcin-1-expressing tumor cells elicits efficient antitumor effects in a mouse model of human lung cancer. Kotaro Abe, Masahiko Kanehira , Shinya Ohkouchi, Sakiko Kumata, Yamato Suzuki, Hisashi Oishi, Masafumi Noda, Akira Sakurada, Eisaku Miyauchi, Tohru Fujiwara, Hideo Harigae, Yoshinori Okada. Cancer medicine. 2021 May;;10:3085-3100. PubMed: 33826244 PMC: PMC8085941