Monoclonal Mouse Biotin Antibody
Catalog Number: LS-C127042-200
200 µg / $755.00
Antibody: Mouse Monoclonal
Applications: Enzyme-Linked Immunosorbent Assay
Format: Liquid
Anti-MADCAM1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC-plus™ Monoclonal Mouse anti-Human MAdCAM-1 Antibody (clone 314G8, Low Endotoxin, IHC)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin |
| Reactivity: | Human |
| Format: | Liquid |
$567.00each
Catalog Number: LS-B3316-0.1
Catalog Number: LS-B3316
For bulk orders or custom formulations:
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- Description
- MAdCAM-1 antibody LS-B3316 is an unconjugated mouse monoclonal antibody to human MAdCAM-1. Validated for ELISA, Flow and IHC. Tested on 20 paraffin-embedded human tissues.
- Application
- ELISA, FC, IHC, IHC-F, IHC-P
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- HMAdCAM-1 | MACAM1 | MAdCAM-1 | MADCAM1
- Recommended Dilution: FC
- 1:10 - 1:100
- Recommended Dilution: IHC-F
- 1:25 - 1:200
- Recommended Dilution: IHC-P
- 10 µg/ml
- UniProt Number
- Q13477
- NCBI GenBank
- AAC51354.1 | NM_130762
- SwissProt
- Q13477
Target
- Target
- MAdCAM-1
- Antigen Species
- Human
- Specificity
- Recognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1), a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyers patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria but clone 314G8 does not recognize MAdCAM-1 in these tissues. Clone Reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognized by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7
- Epitope
- Recognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1), a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyers patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria but clone 314G8 does not recognize MAdCAM-1 in these tissues. Clone Reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognized by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7.
- Gene Name
- MADCAM1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 314G8
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant soluble MAdCAM-1-fc fusion protein
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS
- Concentration
- 1 mg/mL
- Animal Free
- No
- Carrier-Free
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C. Store undiluted. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
- Recommended Storage Buffer
- PBS