LSBio
IHC-plus™ Monoclonal Mouse anti-Human HLA-E Antibody (clone MEM-E/07, IHC)

Anti-HLA-E antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ Monoclonal Mouse anti-Human HLA-E Antibody (clone MEM-E/07, IHC)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation
Reactivity:Human
Format:Liquid
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$383.00each
Catalog Number: LS-B3730-0.05

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Overview

Description
HLA-E antibody LS-B3730 is an unconjugated mouse monoclonal antibody to human HLA-E. Validated for Flow, IHC and IP. Tested on 20 paraffin-embedded human tissues.
Application
FC, IHC, IHC-P, IP
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
EA2.1 | HLAE | HLA-E | MHC HLA-E alpha-2.1 | MHC HLA-E alpha-1 | EA1.2 | HLA-6.2 | MHC class I antigen E | QA1
Recommended Dilution: FC
1 - 4 µg/ml
Recommended Dilution: IHC-P
10 µg/ml
UniProt Number
P13747
NCBI GenBank
NM_005516 | NP_005507.3
SwissProt
P13747

Target

Target
HLA-E
Antigen Species
Human
Specificity
The antibody MEM-E/07 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).
Epitope
The antibody MEM-E/07 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).
Gene Name
HLA-E

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
MEM-E/07
Origin Species
Human

Immunogen

Immunogen
Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 15 mM Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at 2°C to 8°C. Do not freeze.
Recommended Storage Buffer
PBS

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.