LSBio
IHC-plus™ HLA-E Antibody (clone MEM-E/06)

Anti-HLA-E antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ HLA-E Antibody (clone MEM-E/06)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation
Reactivity:Human, Non-Human Primate
Format:Liquid
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$383.00each
Catalog Number: LS-B3729-0.05

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Overview

Description
HLA-E antibody LS-B3729 is an unconjugated mouse monoclonal antibody to HLA-E from human. It is reactive with human and non-human primates. Validated for Flow, IHC and IP. Tested on 20 paraffin-embedded human tissues.
Application
FC, IHC, IHC-P, IP
Reactivity
Hu, NHP
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
EA2.1 | HLAE | HLA-E | MHC HLA-E alpha-2.1 | MHC HLA-E alpha-1 | EA1.2 | HLA-6.2 | MHC class I antigen E | QA1
Recommended Dilution: IHC-P
10 µg/ml
UniProt Number
P13747
SwissProt
P13747

Target

Target
HLA-E
Antigen Species
Human
Specificity
The antibody MEM-E/06 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results showed that the antibody cross-reacts with some classical MHC Class I molecules (HLA-A3, -A11, -B7). However, the recent Workshop I Session on the 3rd International Conference on HLA-G (Paris, July 2003) confirmed that the antibody exhibits much broader cross-reactivity classical MHC Class I antigens, namely with HLA-A24, -A32, -B8, -B15, -B27, -B35, -B44, -B54, -C3, -C4, -C5, -C7.
Epitope
The antibody MEM-E/06 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results showed that the antibody cross-reacts with some classical MHC Class I molecules (HLA-A3, -A11, -B7). However, the recent Workshop I Session on the 3rd International Conference on HLA-G (Paris, July 2003) confirmed that the antibody exhibits much broader cross-reactivity classical MHC Class I antigens, namely with HLA-A24, -A32, -B8, -B15, -B27, -B35, -B44, -B54, -C3, -C4, -C5, -C7.
Gene Name
HLA-E

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
MEM-E/06
Origin Species
Human

Immunogen

Immunogen
Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 15 mM Sodium Azide
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at 2°C to 8°C. Do not freeze.
Recommended Storage Buffer
PBS

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