Anti-HIF3A antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ HIF3A / HIF3-Alpha Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Format:	Liquid

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$526.00each

Catalog Number: LS-B714-50

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Overview

Description: HIF3-Alpha antibody LS-B714 is an unconjugated rabbit polyclonal antibody to mouse HIF3-Alpha (HIF3A). Validated for ELISA, IHC and WB.
Application: ELISA, IHC, IHC-P, WB
Predicted Reactivity: Mouse, Rat
Nonreactive Species: Human
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: BHLHe17 | HIF-3A | IPAS | HIF3-alpha-1 | HIF3A | Inhibitory PAS domain protein | PASD7 | HIF-3-alpha | HIF3-alpha | Member of PAS protein 7 | MOP7
Recommended Dilution: ELISA: 1:14000 - 1:80000
Recommended Dilution: IHC-P: 5 µg/ml
Recommended Dilution: WB: 1:1000 - 1:8000
UniProt Number: Q9Y2N7
NCBI GenBank: NM_152795 | NP_690008.2
SwissProt: Q9Y2N7

Target

Target: HIF3A / HIF3-Alpha
Antigen Species: Mouse
Specificity: A BLAST analysis was used to suggest cross reactivity with Hif3a protein from mouse (100% homology) and rat (91% homology). Reactivity against human Hif3a is not expected as only 75% homology for the immunizing sequence is noted. No reactivity is expected against other forms of Hif proteins. Reactivity against homologues from other sources is not known.
Epitope: A BLAST analysis was used to suggest cross reactivity with Hif3a protein from mouse (100% homology) and rat (91% homology). Reactivity against human Hif3a is not expected as only 75% homology for the immunizing sequence is noted. No reactivity is expected against other forms of Hif proteins. Reactivity against homologues from other sources is not known.
Gene Name: HIF3A

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Origin Species: Human

Immunogen

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 575-600 of mouse Hif3 a (Hypoxia Inducible Factor).
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1.2 mg/mL
Animal Free: No
Carrier-Free: Yes
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Recommended Storage Buffer: 0.02 M Potassium Phosphate, 0.15 M NaCl