LSBio
IHC-plus™ F2RL1 / PAR2 Antibody (N-Terminus, clone SAM11)

Anti-F2RL1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

IHC-plus™ F2RL1 / PAR2 Antibody (N-Terminus, clone SAM11)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin
Reactivity:Human
Format:Liquid
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$495.00each
Catalog Number: LS-B2321-50

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Overview

Description
PAR2 antibody LS-B2321 is an unconjugated mouse monoclonal antibody to human PAR2 (F2RL1) (N-Terminus). Validated for ELISA, IF and IHC. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application
ELISA, IF, IHC, IHC-P
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
F2RL1 | PAR2 | Thrombin receptor-like 1 | Protease-activated receptor 2 | G-protein coupled receptor 11 | GPR11 | PAR-2
Recommended Dilution: ELISA
0.1 - 1 µg/ml
Recommended Dilution: IF/ICC
1 - 3 µg/ml
Recommended Dilution: IHC-P
5 µg/ml
UniProt Number
P55085
SwissProt
P55085

Target

Target
F2RL1 / PAR2
Antigen Species
Human
Specificity
Recognizes native peptide and the N-terminus of the human PAR2 receptor. Does not cross-react with PAR1.
Epitope
Recognizes native peptide and the N-terminus of the human PAR2 receptor. Does not cross-react with PAR1.
Gene Name
F2RL1

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
SAM11
Origin Species
Human

Immunogen

Immunogen
Synthetic peptide corresponding to the N-Terminus of human PAR2 receptor.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 0.09% Sodium Azide
Concentration
0.5 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS
PAR2-dependent activation of GSK3ß regulates the survival of colon stem/progenitor cells. Nasri I, Bonnet D, Zwarycz B, d'Aldebert E, Khou S, Mezghani-Jarraya R, Quaranta M, Rolland C, Bonnart C, Mas E, Ferrand A, Cenac N, Magness S, Van Landeghem L, Vergnolle N, Racaud-Sultan C. American journal of physiology. Gastrointestinal and liver physiology. 2016;311:G221-36. PubMed: 27313176 PMC: PMC5007290

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