Anti-EIF4EBP1 / 4EBP1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ EIF4EBP1 / 4EBP1 Antibody (aa101-118)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunohistochemistry (general)
Reactivity:	Hamster, Human, Mouse, Rabbit, Rat
Format:	Liquid

$383.00each

Catalog Number: LS-B3748-50

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Overview

Description: 4EBP1 antibody LS-B3748 is an unconjugated rabbit polyclonal antibody to 4EBP1 (EIF4EBP1) (aa101-118) from human. It is reactive with human, mouse, rat and other species. Validated for GS, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application: IHC
Reactivity: Ham, Hu, Ms, Rb, Rt
Predicted Reactivity: Bovine, Dog, Goat, Horse, Mammal (Other), Non-Human Primate
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: 4E-BP1 | 4EBP1 | BP-1 | EIF4EBP1 | PHAS-I | EIF4E-binding protein 1
Recommended Dilution: IHC-P: 5 - 10 µg/ml
Recommended Dilution: WB: 4 µg/ml
UniProt Number: Q13541
NCBI GenBank: NM_004095 | NP_004086.1
SwissProt: Q13541

Target

Target: EIF4EBP1 / 4EBP1
Antigen Species: Human
Specificity: Detects multiple bands at 19-25 kD, corresponding to the apparent molecular mass of the various phosphorylation states of PHAS-I protein on SDS-PAGE immunoblots. This antibody provides a simple read-out for PKB (Akt) activity in vivo through the use of gel-shift assays. Antibody specificity has been confirmed in Western blot peptide competition studies.
Epitope: Detects multiple bands at 19-25 kD, corresponding to the apparent molecular mass of the various phosphorylation states of PHAS-I protein on SDS-PAGE immunoblots. This antibody provides a simple read-out for PKB (Akt) activity in vivo through the use of gel-shift assays. Antibody specificity has been confirmed in Western blot peptide competition studies.
Gene Name: EIF4EBP1

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Origin Species: Human

Immunogen

Immunogen: An 18 residue synthetic peptide SPEDKRAGGEESQFEMDI based on the human PHAS-I (aa 101-118) and the peptide coupled to KLH. This sequence is identical to rat and mouse PHAS- I over these residues. Percent identity by BLAST analysis: Human, Gorilla, Mouse, Rat, Hamster, Rabbit (100%); Marmoset, Goat, Elephant, Dog, Bat, Bovine, Horse (94%); Panda (83%).
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 25 mM Sodium Borate, BBS, pH 8.4, 75 mM NaCl, 5 mM EDTA, 100 mM Boric acid
Concentration: 0.9 mg/mL
Animal Free: No
Carrier-Free: Yes
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
Recommended Storage Buffer: 25 mM Sodium Borate, BBS, 75 mM NaCl