Detection of 4EBP1 by Western Blot. Samples: Whole cell lysate (100 ug) from RINm5F insulinoma exposed to high or low concentration of leucine. Antibody: Affinity purified rabbit anti-4EBP1 used at 0.25 ug/ml. Detection: Chemiluminescence.
EIF4EBP1 / 4EBP1 Antibody (aa75-C-term)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C287384-10
Catalog Number: LS-C287384
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- Description
- 4EBP1 antibody LS-C287384 is an unconjugated rabbit polyclonal antibody to human 4EBP1 (EIF4EBP1) (aa75-C-term). Validated for IHC and WB.
- Application
- IHC, WB
- Reactivity
- Hu
- Predicted Reactivity
- Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- 4E-BP1 | 4EBP1 | BP-1 | EIF4EBP1 | PHAS-I | EIF4E-binding protein 1
- Recommended Dilution: IHC
- 1:5000 - 1:20000
- Recommended Dilution: WB
- 1:1000 - 1:6000
- UniProt Number
- Q13541
- NCBI GenBank
- NM_004095 | NP_004086.1
- SwissProt
- Q13541
Target
- Target
- Human EIF4EBP1 / 4EBP1
- Antigen Species
- Human
- Epitope
- Region between residue 75 and the C-terminus (residue 118) of human eukaryotic translation initiation factor 4E binding protein 1 using the numbering given in entry NP_004086.1 (GeneID 1978).
- Gene Name
- EIF4EBP1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 1978 corresponding to amino acids between 75 and the C-Terminus of UniprotID: Q13541
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- Tris-citrate