LSBio
IHC-plus™ CD34 Antibody (clone QBEND-10, Concentrated)

Anti-CD34 antibody IHC of human kidney, glomeruli. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:800.

IHC-plus™ CD34 Antibody (clone QBEND-10, Concentrated)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
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$587.00each
Catalog Number: LS-B5552-50

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Overview

Description
CD34 antibody LS-B5552 is an unconjugated mouse monoclonal antibody to human CD34. Validated for Flow, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Application
FC, IHC, IHC-F, IHC-P, IP, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
CD34 | CD34 molecule | CD34 antigen | HPCA1
Recommended Dilution: IHC-P
1:800
UniProt Number
P28906
SwissProt
P28906

Target

Target
CD34
Antigen Species
Human
Specificity
The antibody recognizes the CD34 antigen, some cross reactions with vascular associated adventitia and some basement membranes. Ultrastructure studies, using an indirect immunogold technique on ultra thin sections of human umbilical artery, have revealed that this antigen is associated to a degree with the basoluminal surfaces of endothelium and shows a slight reaction with an intracytoplasmic vesicular compartment. Predominant staining is on the endothelial cell membrane. QBEND-10 immunoprecipitates a glycoprotein with a relative MW 110kD expressed on hematopoietic cells and on the established myeloid leukemic cell line KG1A. QBEND-10 recognizes the Class II epitope on CD34. This antibody does not cross-react with tested species such as sheep, cow, dog and rat. Clone is reported to cross react with Rhesus Monkey (2). Immunogen: human endothelial vesicles.
Epitope
The antibody recognizes the CD34 antigen, some cross reactions with vascular associated adventitia and some basement membranes. Ultrastructure studies, using an indirect immunogold technique on ultra thin sections of human umbilical artery, have revealed that this antigen is associated to a degree with the basoluminal surfaces of endothelium and shows a slight reaction with an intracytoplasmic vesicular compartment. Predominant staining is on the endothelial cell membrane. QBEND-10 immunoprecipitates a glycoprotein with a relative MW 110kD expressed on hematopoietic cells and on the established myeloid leukemic cell line KG1A. QBEND-10 recognizes the Class II epitope on CD34. This antibody does not cross-react with tested species such as sheep, cow, dog and rat. Clone is reported to cross react with Rhesus Monkey (2). Immunogen: human endothelial vesicles.
Gene Name
CD34

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
QBEND-10
Origin Species
Human

Immunogen

Immunogen
Human endothelial vesicles.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Other

Storage & Handling

Storage Conditions
Store at 2-8°C.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.