LSBio
IHC-plus™ CCL8 / MCP2 Antibody

Anti-MCP-2 / CCL8 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.

IHC-plus™ CCL8 / MCP2 Antibody

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Lyophilized
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$470.00each
Catalog Number: LS-B8198-500

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Overview

Description
MCP2 antibody LS-B8198 is an unconjugated mouse monoclonal antibody to human MCP2 (CCL8). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
C-C motif chemokine 8 | CCL8 | HC14 | MCP2 | Monocyte chemotactic protein 2 | Scya10 | SCYA8 | Small-inducible cytokine A8 | MCP-2 | Chemokine (C-C motif) ligand 8
Recommended Dilution: ELISA
2 - 4 µg/ml
Recommended Dilution: IHC-P
10 µg/ml
Recommended Dilution: WB
0.25 - 0.5 µg/ml
UniProt Number
P80075
SwissProt
P80075

Target

Target
CCL8 / MCP2
Antigen Species
Human
Specificity
Human MCP-2
Epitope
Human MCP-2
Gene Name
CCL8

Antibody

Host Species
Mouse
Clonality
Monoclonal
Light Chain
Kappa Light Chain
Origin Species
Human

Immunogen

Immunogen
E.coli derived recombinant Human MCP-2 cprresponding to amino acids 24-99 of P80075.

Format

Conjugate/Label
Unconjugated
Format
Lyophilized
Formulation
Lyophilized from PBS
Animal Free
No
Carrier-Free
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Recommended Storage Buffer
PBS
Iron accelerates Fusobacterium nucleatum-induced CCL8 expression in macrophages and is associated with colorectal cancer progression. Taishi Yamane, Yohei Kanamori, Hiroshi Sawayama, Hiromu Yano, Akihiro Nita, Yudai Ohta, Hironori Hinokuma, Ayato Maeda, Akiko Iwai, Takashi Matsumoto, Mayuko Shimoda, Mayumi Niimura, Shingo Usuki, Noriko Yasuda-Yoshihara, Masato Niwa, Yoshifumi Baba, Takatsugu Ishimoto, Yoshihiro Komohara, Tomohiro Sawa, Tasuku Hirayama, Hideo Baba, Toshiro Moroishi. JCI insight. 2022 November;7. PubMed: 36136589 PMC: PMC9675438

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