LSBio
IHC-plus™ APOB / Apolipoprotein B Antibody

Anti-Apolipoprotein B antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ APOB / Apolipoprotein B Antibody

Primary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
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$699.00each
Catalog Number: LS-B3646-50

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Overview

Description
Apolipoprotein B antibody LS-B3646 is an unconjugated goat polyclonal antibody to human Apolipoprotein B (APOB). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IHC, IHC-P, IP, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ApoB-100 | APOB | Apolipoprotein B-48 | ApoB-48 | Apolipoprotein B | FLDB | LDLCQ4 | Mutant Apo B 100 | Apo B-100 | Apolipoprotein B-100 | Apolipoprotein B48
Recommended Dilution: ELISA
1:2000 - 1:10000
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: IP
1:100
Recommended Dilution: WB
1:200 - 1:1000
UniProt Number
P04114
SwissProt
P04114

Target

Target
APOB / Apolipoprotein B
Antigen Species
Human
Specificity
Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
Epitope
Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
Gene Name
APOB

Antibody

Host Species
Goat
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
ApoLipoprotein Type B was isolated from human plasma by density gradient centrifugation followed by HPLC purification

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.125 M Sodium Borate, pH 8.0, 0.005 M EDTA, 0.075 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Recommended Storage Buffer
0.125 M Sodium Borate, 0.075 M NaCl

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