Immunohistochemistry Dilution at 1:600 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
IGHM / IgM Antibody
Secondary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$300.00each
Catalog Number: LS-C680644-50
Catalog Number: LS-C680644
For bulk orders or custom formulations:
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- Description
- IgM antibody LS-C680644 is an unconjugated rabbit polyclonal antibody to human IgM (IGHM). Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Secondary Antibodies
- Alternative Names
- AGM1 | IGM | MU | VH | IGHM
- UniProt Number
- P01871
- SwissProt
- P01871
Target
- Target
- Human IGHM / IgM
- Antigen Species
- Human
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant Human Immunoglobulin heavy constant mu protein (45-178AA)
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
- Recommended Storage Buffer
- 0.01 M PBS