IHC analysis of IGFBP2 using anti-IGFBP2 antibody. IGFBP2 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2?g/ml rabbit anti-IGFBP2 Antibody overnight at 4?C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IGFBP2 / IGF-BP53 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Mouse, Rat |
| Format: | Lyophilized |
$480.00each
Catalog Number: LS-C756478-100
Catalog Number: LS-C756478
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- Description
- IGF-BP53 antibody LS-C756478 is an unconjugated rabbit polyclonal antibody to IGF-BP53 (IGFBP2) from rat. It is reactive with mouse and rat. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC-P, WB
- Reactivity
- Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BP2 | IGFBP-2 | IGF-binding protein 2 | IBP-2 | IGFBP2 | IBP2 | IGF-BP53
- Recommended Dilution: ELISA
- 0.1 - 0.5 ug/ml
- Recommended Dilution: IHC-P
- 0.5 - 1 µg/ml
- Recommended Dilution: WB
- 0.1 - 0.5 µg/ml
- UniProt Number
- P18065
- NCBI GenBank
- NM_000597 | NP_000588.2
- SwissProt
- P18065
Target
- Target
- Rat IGFBP2 / IGF-BP53
- Antigen Species
- Rat
- Epitope
- No cross reactivity with other proteins.
- Gene Name
- IGFBP2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- E. coli-derived rat IGFBP2 recombinant protein (Position: E35-Q304).
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
- Recommended Storage Buffer
- NaCl