Immunofluorescence staining of Mouse ICAM1 in Raw264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse ICAM1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Cell membrane.
ICAM-1 / CD54 Antibody (clone 101, Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence |
| Reactivity: | Mouse |
| Format: | Liquid |
$164.00each
Catalog Number: LS-C819332-20
Catalog Number: LS-C819332
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- Description
- CD54 antibody LS-C819332 is an unconjugated rabbit monoclonal antibody to mouse CD54 (ICAM-1). Validated for ELISA, ICC and IF.
- Application
- ELISA, ICC, IF
- Reactivity
- Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- BB2 | CD54 antigen | CD54 | ICAM-1 | ICAM1 | p3.58
- Recommended Dilution: ELISA
- 1:1000 - 1:10000
- Recommended Dilution: IF/ICC
- 1:20 - 1:100
- UniProt Number
- P05362
- NCBI GenBank
- NM_000201 | NP_000192.2
- SwissProt
- P05362
Target
- Target
- Mouse ICAM-1 / CD54
- Antigen Species
- Mouse
- Epitope
- Mouse ICAM-1
- Gene Name
- ICAM1
Antibody
- Host Species
- Rabbit
- Clonality
- Monoclonal
- Clone
- 101
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Mouse ICAM-1 / CD54 Protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 20 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS