1:100 staining human appendix tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ICAM-1 / CD54 Antibody (phospho-Tyr512)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Non-Human Primate |
| Format: | Liquid |
$288.00each
Catalog Number: LS-C800948-100
Catalog Number: LS-C800948
For bulk orders or custom formulations:
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- Description
- CD54 antibody LS-C800948 is an unconjugated rabbit polyclonal antibody to CD54 (ICAM-1) (pTyr512) from human. It is reactive with human, mouse and monkey. Validated for IF, IHC, Peptide-ELISA and WB.
- Application
- ELISA, IF, IHC, WB
- Reactivity
- Hu, Ms, NHP
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BB2 | CD54 antigen | CD54 | ICAM-1 | ICAM1 | p3.58
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: IHC
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P05362
- NCBI GenBank
- NM_000201 | NP_000192.2
- SwissProt
- P05362
Target
- Target
- Human ICAM-1 / CD54
- Antigen Species
- Human
- Epitope
- Phospho-ICAM-1 (Tyr512) Antibody detects endogenous levels of ICAM-1 only when phosphorylated at Tyrosine 512.
- Gene Name
- ICAM1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthesized peptide derived from human ICAM-1 around the phosphorylation site of Tyrosine 512.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS