Immunocytochemistry of rabbit Anti-Histone H3 [Monomethyl Lys4] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [Monomethyl Lys4] antibody at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [Monomethyl Lys4] is nuclear and chromosomal. Staining: Histone H3 [Monomethyl Lys4] is expressed in green while the nuclei and aplpha-tubulin were coexpressed with DAPI (blue) and Phalloidin (red).

Histone H3 Antibody (Me-Lys4)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Chromatin Immunoprecipitation, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:	Chicken, Fruit Fly, Helminth / Nematode, Human, Mouse, Plant, Rat, Xenopus
Format:	Liquid

$594.00each

Catalog Number: LS-C745440-50

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: Histone H3 antibody LS-C745440 is an unconjugated rabbit polyclonal antibody to Histone H3 (Me-Lys4) from human. It is reactive with human, mouse, rat and other species. Validated for ChrIP, IF, IHC and WB.
Application: ChIP, IF, IHC, WB
Reactivity: Ck, Dros, Helm, Hu, Ms, Plnt, Rt, Xen
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: IF/ICC: 1:500
Recommended Dilution: IHC: 1:500
Recommended Dilution: IP: 2 - 5 µg/10E6 cells
Recommended Dilution: WB: 1:500

Target

Target: Human Histone H3
Antigen Species: Human
Epitope: This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Histone H3 [Monomethyl Lys4] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Lysine 4 of human Histone H3.2.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide, 30% Glycerol
Concentration: 0.72 mg/mL
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl