Western Blot of rabbit anti-Histone H3 K4Me1 antibody. Lane 1: Raji Whole Cell Lysate. Load: 35 µg per lane. Primary antibody: H3K4me1 antibody at 1:2000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:5,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15kDa for Histone H3.

Histone H3 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Chromatin Immunoprecipitation, Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:	Human
Format:	Liquid

$311.00each

Catalog Number: LS-C745012-25

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: Histone H3 antibody LS-C745012 is an unconjugated rabbit polyclonal antibody to human Histone H3. Validated for ChrIP, ELISA, IF, IHC and WB.
Application: ChIP, ELISA, IF, IHC, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA: 1:3000 - 1:10000
Recommended Dilution: IF/ICC: 2 - 5 µg/ml
Recommended Dilution: IHC: 2 - 5 µg/ml
Recommended Dilution: IP: 2 - 5 µg/10E6 cells
Recommended Dilution: WB: 1:500 - 1:2000

Target

Target: Human Histone H3
Antigen Species: Human
Epitope: This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Histone H3 K4me1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Lysine 4 of human Histone H3.2.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Volume: 25 Microliter
Preservative: Yes
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl