CD276 / B7-H3 Antibody
Catalog Number: LS-C394162-100
100 µL / $394.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human
Format: Liquid
Dot Blot of Rabbit Histone H3 K27 me1-3 Antibody. Lane 1: Unmodified. Lane 2: me1. Lane 3: me2. Lane 4: me3. Lane 5: ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [1-3 methyl, Lys27, ac] antibody at 0.5 ug/ml for 45 min at 4 degrees C. Secondary antibody: Dylight alpha 488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4 degrees C.
Histone H3 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Dot Blot, Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Helminth / Nematode, Human, Mouse, Rat |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C209444-50
Catalog Number: LS-C209444
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- Histone H3 antibody LS-C209444 is an unconjugated rabbit polyclonal antibody to Histone H3 from human. It is reactive with human, mouse, rat and other species. Validated for DB, ELISA and WB.
- Application
- DB, ELISA, WB
- Reactivity
- Helm, Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Recommended Dilution: WB
- 1:1000
Target
- Target
- Human Histone H3
- Antigen Species
- Human
- Epitope
- This antibody recognizes the Me1, Me2 and Me3 of Lys27 for human Histone H3. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, rat, and C. elegans based on 100% sequence homology. Cross-reactivity with Histone H3 [methyl Lys27] from other sources has not been determined.
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Histone H3 K27 methyl Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the K27 me3 site of human Histone H3.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 0.83 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
