LSBio
His Tag Antibody

Anti-6X His Antibody - Western Blot. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal rabbit.

His Tag Antibody

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Format:Liquid
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$429.00each
Catalog Number: LS-C60077-100

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Overview

Description
His Tag antibody LS-C60077 is an unconjugated rabbit polyclonal antibody to His Tag. Validated for ELISA, IHC and WB. Cited in 1 publication.
Application
ELISA, IHC, WB
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:10000 - 1:40000
Recommended Dilution: IHC
1:500 - 1:2000
Recommended Dilution: WB
1:500 - 1:1000

Target

Target
His Tag
Epitope
This antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
This antibody was purified from whole rabbit serum prepared by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1.22 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl
Single amino acid substitutions in the severe acute respiratory syndrome coronavirus spike glycoprotein determine viral entry and immunogenicity of a major neutralizing domain. Yi CE, Ba L, Zhang L, Ho DD, Chen Z. Journal of virology. 2005;79:11638-46. PubMed: 16140741 PMC: PMC1212612
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