LSBio
Monoclonal Mouse His Tag Antibody (clone 33D10.D2.G8, WB)

Anti-6x-His Epitope Tag Monoclonal Antibody - Western Blot. Western blot of Immunochemicals Mouse Anti-6x-His Epitope Tag Monoclonal Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). In lane 1 are molecular weight markers.

Monoclonal Mouse His Tag Antibody (clone 33D10.D2.G8, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot
Format:Liquid
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$452.00each
Catalog Number: LS-C153999-100

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Overview

Description
His Tag antibody LS-C153999 is an unconjugated mouse monoclonal antibody to His Tag. Validated for ELISA, IP and WB.
Application
ELISA, IP, WB
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: ELISA
1:75000
Recommended Dilution: IP
1:200
Recommended Dilution: WB
1:1000

Target

Target
His Tag
Epitope
This antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
33D10.D2.G8
Isotype
IgG1
Light Chain
Kappa Light Chain

Immunogen

Immunogen
HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1.09 mg/mL
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.