LSBio
Goat Anti-Human IgG Secondary Antibody (HRP)

ELISA results of purified Goat anti-Human IgG Antibody Peroxidase conjugated tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 1.0 µg of Human IgG The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate

Goat Anti-Human IgG Secondary Antibody (HRP)

Secondary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Other, Western Blot
Reactivity:Human
Format:Lyophilized
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$349.00each
Catalog Number: LS-C60543-2

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Overview

Description
Human IgG antibody LS-C60543 is an HRP-conjugated goat polyclonal antibody. Validated for DB, ELISA, EM, IHC and WB.
Application
DB, ELISA, IHC, Other, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:20000 - 1:80000
Recommended Dilution: IHC
1:1000 - 1:5000
Recommended Dilution: WB
1:5000 - 1:25000

Target

Target
Human IgG
Antigen Species
Human
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG and Human Serum.

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Human IgG (H&L) was produced by repeated immunization with human IgG whole molecule in goat.
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Horseradish Peroxidase
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Concentration
2 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.