LSBio
Mouse Anti-Human IgG Secondary Antibody (FITC)

Fluorescein Mouse Anti-Human IgG (H&L) Antibody - Dot Blot. Dot Blot showing the detection of Human IgG. A three-fold serial dilution of Human IgG starting at 200 ng was spotted onto 0.45 um nitrocellulose. After blocking in 5% Blotto (B501-0500) 1 Hour at 20?, Anti-Human IgG (H&L) (MOUSE) Antibody Fluorescein Conjugated Anti-HUMAN IgG (H&L) (MOUSE) Antibody Fluorescein Conjugated - 609-302-002 secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blot (p/n MB-070) and imaged using the Bio-Rad VersaDoc 4000 MP. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Mouse Anti-Human IgG Secondary Antibody (FITC)

Secondary AntibodyLSBio Guarantee
Antibody:Mouse Polyclonal
Applications:Dot Blot, Flow Cytometry, Immunofluorescence, Other
Reactivity:Human
Format:Lyophilized
See all Human IgG antibodies →
$393.00each
Catalog Number: LS-C154316-1

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Overview

Description
Human IgG antibody LS-C154316 is an FITC-conjugated mouse polyclonal antibody. Validated for DB, FLISA, Flow and IF.
Application
DB, FC, IF, Other
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: FC
1:500 - 1:2500
Recommended Dilution: IF/ICC
1:1000 - 1:5000

Target

Target
Human IgG
Antigen Species
Human
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Mouse Serum, Human IgG and Human Serum.

Antibody

Host Species
Mouse
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Human IgG whole molecule

Format

Conjugate/Label
Fluorescein Isothiocyanate
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.