Western blot analysis of extracts of various cell lines, using ENO1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
ENO1 / Alpha Enolase Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$293.00each
Catalog Number: LS-C331216-20
Catalog Number: LS-C331216
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- Description
- Alpha Enolase antibody LS-C331216 is an unconjugated rabbit polyclonal antibody to Alpha Enolase (ENO1) from human. It is reactive with human and mouse. Validated for IF, IHC, IP and WB.
- Application
- IF, IHC, IP, WB
- Reactivity
- Hu, Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Alpha Enolase | Alpha enolase like 1 | ENO1 | ENO1L1 | Enolase 1 | MBP-1 | MBPB1 | MPB1 | MYC promoter-binding protein 1 | NNE | Phosphopyruvate hydratase | Alpha-enolase | Tau-crystallin | MBP1 | MPB-1 | C-myc promoter-binding protein | Enolase 1, (alpha) | Enolase-alpha | Non-neural enolase | Plasminogen-binding protein | PPH
- Recommended Dilution: IF/ICC
- 1:50 - 1:100
- Recommended Dilution: IHC
- 1:50 - 1:200
- Recommended Dilution: IP
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P06733
- NCBI GenBank
- NM_001428 | NP_001419.1
- SwissProt
- P06733
Target
- Target
- Human ENO1 / Alpha Enolase
- Antigen Species
- Human
- Epitope
- Human ENO1 / Alpha Enolase
- Gene Name
- ENO1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant fusion protein containing a sequence corresponding to amino acids 1-434 of human ENO1 (NP_001419.1). MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVG
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS