Blot of HeLa cell lysates probed with monoclonal antibody ENO1 / Alpha Enolase antibody. The antibody stains a single sharp band corresponding to enolase-1/alpha-enolase/NNE at about 47 kDa.
ENO1 / Alpha Enolase Antibody (clone 253)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Western Blot |
| Reactivity: | Bovine, Human, Mouse, Rat, Xenopus |
| Format: | Liquid |
$384.00each
Catalog Number: LS-C204514-100
Catalog Number: LS-C204514
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- Description
- Alpha Enolase antibody LS-C204514 is an unconjugated mouse monoclonal antibody to Alpha Enolase (ENO1) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF and WB.
- Application
- ICC, IF, WB
- Reactivity
- Bov, Hu, Ms, Rt, Xen
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Alpha Enolase | Alpha enolase like 1 | ENO1 | ENO1L1 | Enolase 1 | MBP-1 | MBPB1 | MPB1 | MYC promoter-binding protein 1 | NNE | Phosphopyruvate hydratase | Alpha-enolase | Tau-crystallin | MBP1 | MPB-1 | C-myc promoter-binding protein | Enolase 1, (alpha) | Enolase-alpha | Non-neural enolase | Plasminogen-binding protein | PPH
- Recommended Dilution: IF/ICC
- 1:2000 - 1:5000|1.43055555555556 - 1:5000
- Recommended Dilution: WB
- 1:5000 - 1:10000
- UniProt Number
- P06733
- NCBI GenBank
- NM_001428 | NP_001419.1
- SwissProt
- P06733
Target
- Target
- Human ENO1 / Alpha Enolase
- Antigen Species
- Human
- Epitope
- Clone 253 is known to react with enolase 1 from human, bovine, mouse, rat and Xenopus. The antibody was tested for binding to expressed bovine enolase 1, 2 and 3 and shown to be specific for only enolase 1.
- Gene Name
- ENO1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 253
- Isotype
- IgG1
- Light Chain
- Kappa Light Chain
Immunogen
- Immunogen
- N-Terminus 12 amino acids of bovine enolase 1 which was synthesized on a 8-amine lysine core using the multiple antigen presentation method. This produces a dendrimer presenting 8 peptides to the immune system.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 10 mM Sodium Azide
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 4°C or -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS