Western blot analysis of extracts of various cell lines, using EGLN1 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.

EGLN1 / PHD2 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Western Blot
Reactivity:	Human, Mouse, Rat
Format:	Liquid

$293.00each

Catalog Number: LS-C749557-20

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Overview

Description: PHD2 antibody LS-C749557 is an unconjugated rabbit polyclonal antibody to PHD2 (EGLN1) from human. It is reactive with human, mouse and rat. Validated for WB.
Application: WB
Reactivity: Hu, Ms, Rt
Intended Use: RUO
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: C1orf12 | EGL9 | EGLN1 | ECYT3 | Egl nine-like protein 1 | HIFPH2 | HIF prolyl hydroxylase 2 | HIF-PH2 | HIF-prolyl hydroxylase 2 | ZMYND6 | PHD2 | SM20 | EGL nine (C.elegans) homolog 1 | Egl nine homolog 1 | HPH-2 | HPH2 | SM-20
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: Q9GZT9
NCBI GenBank: NM_022051, NP_071334.1
SwissProt: Q9GZT9

Target

Target: Human EGLN1 / PHD2
Antigen Species: Hu
Gene Name: EGLN1

Antibody

Host Species: Rb
Clonality: Polyclonal
Isotype: IgG
Origin Species: Hu

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-426 of human EGLN1 (NP_071334.1). MANDSGGPGGPSPSERDRQYCELCGKMENLLRCSRCRSSFYCCKEHQRQDWKKHKLVCQGSEGALGHGVGPHQHSGPAPPAAVPPPRAGAREPRKAAARRDNASGDAAKGKVKAKPPADPAAAASPCRAAAGGQGSAVAAEAEPGKEEPPARSSLFQEKANLYPPSNTPGDALSPGGGLRPNGQTKPLPALKLALEYIVPCMNKHGICVVDDFLGKETGQQIGDEVRALHDTGKFTDGQLVSQKSDSSKDIRGDKITWIEGKEPGCETIGLLMSSMDDLIRHCNGKLGSYKINGRTKAMVACYPGNGTGYVRHVDNPNGDGRCVTCIYYLNKDWDAKVSGGILRIFPEGKAQFADIEPKFDRLLFFWSDRRNPHEVQPAYATRYAI
Immunogen Type: Fusion-P