Western blot analysis of extracts of various cell lines, using EGLN1/EGLN2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
EGLN1 / PHD2 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C497051-20
Catalog Number: LS-C497051
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- Description
- PHD2 antibody LS-C497051 is an unconjugated rabbit polyclonal antibody to human PHD2 (EGLN1). Validated for WB.
- Application
- WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- C1orf12 | EGL9 | EGLN1 | ECYT3 | Egl nine-like protein 1 | HIFPH2 | HIF prolyl hydroxylase 2 | HIF-PH2 | HIF-prolyl hydroxylase 2 | ZMYND6 | PHD2 | SM20 | EGL nine (C.elegans) homolog 1 | Egl nine homolog 1 | HPH-2 | HPH2 | SM-20
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q9GZT9
- NCBI GenBank
- NM_022051 | NP_071334.1
- SwissProt
- Q9GZT9
Target
- Target
- Human EGLN1 / PHD2
- Antigen Species
- Human
- Gene Name
- EGLN1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A synthetic Peptide of human EGLN1/EGLN2
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS