LSBio
EGFR Antibody (clone AT2H8)

Western Blot: Extracts of HaCaT were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EGFR (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

EGFR Antibody (clone AT2H8)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Liquid
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$310.00each
Catalog Number: LS-C175797-50

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Overview

Description
EGFR antibody LS-C175797 is an unconjugated mouse monoclonal antibody to human EGFR. Validated for ELISA, ICC, IF and WB.
Application
ELISA, ICC, IF, IHC, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
EGFR | EGF Receptor | ERBB | ERBB1 | MENA | PIG61 | Proto-oncogene c-ErbB-1 | V-erb-b homolog | EGF-R | HER1
Recommended Dilution: IF/ICC
1:250
Recommended Dilution: WB
1:250
UniProt Number
P00533
SwissProt
P00533

Target

Target
Human EGFR
Antigen Species
Human
Epitope
Human EGFR
Gene Name
EGFR

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
AT2H8
Isotype
IgG2b
Light Chain
Kappa Light Chain

Immunogen

Immunogen
Recombinant human EGFR (424-605aa) purified from E. coli.
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 0.1% Sodium Azide
Concentration
1 mg/mL
Volume
50 Microliter
Preservative
Yes
Purification Method
Protein G Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.