LSBio
IHC-plus™ EGFR Antibody (Internal)

Immunohistochemical analysis of EGFR staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

IHC-plus™ EGFR Antibody (Internal)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Bovine, Chicken, Human, Mouse, Pig, Rat, Zebrafish
Format:Liquid
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$383.00each
Catalog Number: LS-B16091-50

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Overview

Description
EGFR antibody LS-B16091 is an unconjugated rabbit polyclonal antibody to EGFR (Internal) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ICC, IF, IHC, IHC-P, WB
Reactivity
Bov, Ck, Hu, Ms, Por, Rt, Zf
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
EGFR | EGF Receptor | ERBB | ERBB1 | MENA | PIG61 | Proto-oncogene c-ErbB-1 | V-erb-b homolog | EGF-R | HER1
Recommended Dilution: IF/ICC
1:100 - 1:500
Recommended Dilution: IHC-P
1:100
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
P00533
NCBI GenBank
NM_005228 | NP_005219.2
SwissProt
P00533

Target

Target
EGFR
Antigen Species
Human
Specificity
Recognizes endogenous levels of EGFR protein.
Epitope
Recognizes endogenous levels of EGFR protein.
Gene Name
EGFR

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human EGFR.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Concentration
1 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Recommended Storage Buffer
PBS

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