HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L.
DOT1L / DOT1 Antibody (aa1008-1424, clone OTI6E7)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$443.00each
Catalog Number: LS-C336653-100
Catalog Number: LS-C336653
For bulk orders or custom formulations:
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- Description
- DOT1 antibody LS-C336653 is an unconjugated mouse monoclonal antibody to human DOT1 (DOT1L) (aa1008-1424). Validated for IHC and WB.
- Application
- IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- DOT1L | DOT1 | DOT1-like protein | KMT4 | Lysine N-methyltransferase 4 | KIAA1814 | H3-K79-HMTase
- Recommended Dilution: IHC-P
- 1:150
- Recommended Dilution: WB
- 1:2000
- UniProt Number
- Q8TEK3
- NCBI GenBank
- NP_115871.1
- SwissProt
- Q8TEK3
Target
- Target
- Human DOT1L / DOT1
- Antigen Species
- Human
- Epitope
- Human DOT1L / DOT1.
- Gene Name
- DOT1L
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- OTI6E7
- Isotype
- IgG2b
Immunogen
- Immunogen
- Human recombinant protein fragment corresponding to amino acids 1008-1424 of human DOT1L (NP_115871) produced in E. coli.
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS