Detection of Human DOT1L by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-DOT1L used at a dilution of 1:100. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.
DOT1L / DOT1 Antibody (aa1000-1050)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general) |
| Reactivity: | Human |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C286711-1
Catalog Number: LS-C286711
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- Description
- DOT1 antibody LS-C286711 is an unconjugated rabbit polyclonal antibody to human DOT1 (DOT1L) (aa1000-1050). Validated for IHC.
- Application
- IHC
- Reactivity
- Hu
- Predicted Reactivity
- Non-Human Primate
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- DOT1L | DOT1 | DOT1-like protein | KMT4 | Lysine N-methyltransferase 4 | KIAA1814 | H3-K79-HMTase
- Recommended Dilution: IHC
- 1:100 - 1:500
- UniProt Number
- Q8TEK3
- NCBI GenBank
- NP_115871.1
- SwissProt
- Q8TEK3
Target
- Target
- Human DOT1L / DOT1
- Antigen Species
- Human
- Epitope
- Region between residue1000 and 1050 of human DOT1 Like using the numbering given in entry NP_115871.1 (GeneID 84444).
- Gene Name
- DOT1L
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 84444 corresponding to amino acids between 1000 and 1050 of UniprotID: Q8TEK3
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- TBS, 0.09% Sodium Azide, 0.1% BSA
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- TBS