Western Blot results of Rabbit Anti-Deoxyribonuclease 1 Antibody. Lane 1: Opal Prestained Molecular Weight Ladder Lane 2: Deoxyribonuclease 1. Load: 50ng. Primary Antibody: Rabbit Anti-Deoxyribonuclease 1 at 1µg/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP at 1:70,000 for 30min at RT.BlockOut for 30 min at RT.
DNASE1 / DNase I Antibody (Lyophilized)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot |
| Reactivity: | Bovine |
| Format: | Lyophilized |
$579.00each
Catalog Number: LS-C209228-100
Catalog Number: LS-C209228
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- Description
- DNase I antibody LS-C209228 is an unconjugated rabbit polyclonal antibody to bovine DNase I (DNASE1). Validated for ELISA, IP and WB.
- Application
- ELISA, IP, WB
- Reactivity
- Bov
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Deoxyribonuclease I | Deoxyribonuclease i precursor | DNase I | DNL1 | DRNI | Deoxyribonuclease-1 | DNase I, lysosomal | DNASE1
- Recommended Dilution: ELISA
- 1:5000 - 1:20000
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:500 - 1:5000
- UniProt Number
- P24855
- NCBI GenBank
- NM_005223 | NP_005214.2
- SwissProt
- P24855
Target
- Target
- Bovine DNASE1 / DNase I
- Antigen Species
- Bovine
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Deoxyribonuclease A [Bovine Pancreas]. Cross reactivity against Deoxyribonuclease A from other tissues and species may occur but have not been specifically determined.
- Gene Name
- DNASE1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Deoxyribonuclease A [Bovine Pancreas].
Format
- Conjugate/Label
- Unconjugated
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl